Poster
Presentation 17:
Application of Anti-Death Proteins
to Mammalian Cell Culture
Bruno Figueroa Jr, Tina M. Sauerwald,
J. Marie Hardwick, and Michael J. Betenbaugh
Johns Hopkins University
3400 North Charles Street
Baltimore, MD 21218
bruno@jhu.edu
(410) 516-5336
Mammalian cells undergo apoptosis due to various stimuli
common in large-scale protein production schemes. In our research, CHO
and BHK cells have been genetically engineered with anti-death members
of the Bcl-2 family, bcl-2 and bcl-xL. Both CHO and BHK modified cells
were subjected to two model apoptotic stimuli, Sindbis virus and serum
deprivation. Apoptosis was delayed but not completely inhibited by
the overexpression of the anti-death genes. Biological changes occurring
within the cell appear to alter the anti-death proteins’ effectiveness
to inhibit apoptosis.
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