Poster
Presentation 09:
First Principles Modeling of Protein
Folding and Secretion in Saccharomyces cerevisiae
Kenneth J. Kauffman, Anne S. Robinson,
Prasad S. Dhurjati, Francis J. Doyle III
University of Delaware
Department of Chemical Engineering
Newark, DE 19716
kkauffma@udel.edu
(302) 831-6851
Eukaryotic protein folding occurs by
a series of complex reactions. Insight into these reactions can provide
an improved understanding of the limitations to protein folding in the
cell and methods to overcome these limitations. Previously, there have
been few attempts to model all of these steps simultaneously to obtain
an improved understanding of the bottlenecks in protein folding. We have
developed a first principles based, nonlinear model based on the known
biological reactions and regulatory pathways. In portions of the model,
key reactions have not yet been completely identified. In these cases,
mathematical approximations for observed experimental phenomena have been
used. The resulting model provides insight into potential avenues for experimental
improvement of the yeast secretory pathway, improved understanding of the
biological implications of the pathways, indications of potential control
mechanisms, and direction of future sensor development. We will discuss
several preliminary findings from the model and the experiments suggested
from the model outcomes.
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