Poster
Presentation 07:
Protocol Development To Examine The
Effect of Bulk Shear Exposure On Staphylococcus aureus Surface Adhesion
Lisa Mascari and Julie M Ross
Chemical and Biochemical Engineering
University of Maryland, Baltimore
County
Staphylococcus aureus is a pathogenic
organism with antibiotic resistance
that binds to collagen under static
and dynamic conditions. In vivo,
blood-born bacteria are exposed to
shear stress in the bulk phase as they
travel through the vasculature.
For the bacteria to initiate an infection,
the cells must also adhere to the
vascular surface or subendothelium in the
presence of shear stress. This study
attempts to mimic exposure of the cells
to bulk shear in vivo prior to investigating
dynamic surface adhesion. A
constant stress rheometer is used
to expose the cells to bulk shear before
the dynamic adhesion is quantified
using a parallel plate flow chamber, phase
contrast videomicroscopy and digital
imaging processing. The dynamic surface
adhesion results from cells exposed
to bulk shear are compared to dynamic
surface adhesion results of control
cells. The results of this study will be
used to determine whether exposure
to bulk shear stress affects dynamic S.
aureus surface adhesion.
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